Neuroprotective Potential of Vinpocetine in Drosophila Melanogaster Genetic Model of Parkinson Disease
), Olasunmbo O. Afolayan(2), Ismail O. Ishola(3),
(1) Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria.
(2) Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria.
(3)
Corresponding Author
Abstract
Background: Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and dysfunction in both motor and non-motor systems. Current pharmacological treatments are limited by motor complications and do not stop disease progression.
Objectives: This study evaluated the neuroprotective potential of vinpocetine in a genetic Drosophila model of PD expressing human ?-synuclein in dopaminergic neurons, and also its effect on the fecundity and lifespan of Drosophila.
Methods: Transgenic Drosophila melanogaster strains (Elav-GAL4<UAS-syn, DDCGAL4<UAS-syn) and wild-type flies were cultured on normal diet or vinpocetinesupplemented feed at 5, 25, and 50 µM. Fecundity, larva motility, climbing ability, memory performance, and longevity assays were conducted over 28 days. Data were analysed using one way ANOVA and multiple comparison post hoc tests.
Results: Vinpocetine significantly reduced fecundity at 25 and 50 µM. It improved larval motility and climbing activity in a dose-dependent manner. A significant increase in lifespan was observed at 25 µM. Memory assay did not show statistically significant differences across treatment groups.
Conclusion: Vinpocetine demonstrated neuroprotective activity in ?-synucleinexpressing Drosophila by improving motor function and survival. These findings support its potential utility in PD therapy.Keywords
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